- •2) Objects and methods of animal biotechnology
- •3) Totipotent, multipotent, pluripotent animal cells
- •4.Allophenic animals. Genetic chimers
- •5)The principles of genetic cloning
- •6.Allophenic animals. Genetic chimers
- •8) Methods for introducing foreign dna into animal cells
- •9)Cryopreservation of reproductive and germ cells of animals and humans
- •11)The principles and methods of plant cells cultivation in vitro
- •12. The types of medium. Physiological means of compounds medium (as an example you can use the composition of Murashige-Skug medium)
- •14)Differentiation and dedifferentiation in plant cell culture. The obtaining callus mass and cultivation of callus tissue .
- •15)The influence of phytohormons on morphogenesis and regeneration in plant cells culture
- •16.The main path of morphogenesis processes in plant cells culture
- •18.The growth stages in suspension culture
- •20) The factors influenced on microclonal propagation in plant cell culture.
- •21) What is Biotechnology? Various definitions of “Biotechnology”. History of Biotechnology
- •22.Microbial Biotechnology: fundamentals of applied microbiology
- •24.Sterilization in Biotechnology: Methods and principles
- •26) Somaclonal and gametoclonal variation in plant cells culture.
- •27) Artificial seeds". Embryo culture in vitro
- •28. Culture of apical meristem cells
- •29)Cell reconstruction. Theoretical means of cell reconstruction
- •30.Basics of phytopathology. The main diagnostics methods of plant diseases
- •32) Main objects of animal biotechnology:
- •33) Morphological and functional features of gametes - eggs and sperm
- •34Hormonal regulation of mammalian reproduction
- •35)The history of investigations of the genetic transformation of animal cells
- •36.The principles of genetic engineering in animal biotechnology
- •53)Genetic engineering. Methods of genetic transformation
- •54. Methods of receiving plant materials without viruses
- •56) The vector systems used in the genetic engineering
- •57) Methods of genetic engineering: agrobacterial genetic transformation
- •58)Methods of genetic engineering: bioballistics methods
- •60.Apply cell technology and cryopreservation technology for safe gene bank
- •62) Methods of producing chimeras
- •63) Collection and cultivation of oocytes in vivo and in vitro
- •64 Collection and cultivation of embryos in vivo and in vitro
- •66.Fertilization of oocytes in vitro, environment and conditions
- •68) Draw a diagram of the structure of plasmid pBr322
- •69) Draw a diagram of an experiment in genetic engineering (design recDna) and give a description of the main stages
- •70)Describe the calcium-phosphate method for introducing foreign dna into mammalian cells.
- •72 Methods of cryopreservation of sperm and oocytes of mammals
- •74) Modes of freezing and thawing of gametes and embryos
- •75) Methods of artificial fertilization: gamete insemination fallopian tube (gift), zygosity insemination fallopian tubes (zift).
- •76) Stem cells and prospects for their use in practice
- •78.Technical equipment of experiments on artificial insemination
- •80) Methods of animal cloning, reproductive and therapeutic cloning
- •81) Microorganisms in water and wastewater treatment
- •82 Microbial fermentations in food products
- •84.Bacterial examination of water and standard water analysis
- •86) Use of e.Coli for the biotechnological production
- •87) Microbes in milk and dairy products
- •88) What is the benefit of microorganisms in industry
- •90. Algae, their applications
82 Microbial fermentations in food products
Fermentation has always been an important part of our lives: foods can be spoiled by microbial fermentations, foods can be made by microbial fermentations, and muscle cells use fermentation to provide us with quick responses. Fermentation could be called the staff of life because it gives us the basic food, bread.
Fermentation is the process that produces alcoholic beverages or acidic dairy products. For a cell, fermentation is a way of getting energy without using oxygen. In general, fermentation involves the breaking down of complex organic substances into simpler ones. The microbial or animal cell obtains energy through glycolysis, splitting a sugar molecule and removing electrons from the molecule. The electrons are then passed to an organic molecule such as pyruvic acid. This results in the formation of a waste product that is excreted from the cell. Waste products formed in this way include ethyl alcohol, butyl alcohol, lactic acid, and acetone--the substances vital to our utilization of fermentation.
Microbial fermentations have other benefits. For one, they don't use toxic reagents or require the addition of intermediate reagents.
Fermentation is one way microorganisms can change a food. Yeast, especially Saccharomyces cerevisiae, is used to leaven bread, brew beer and make wine. Certain bacteria, including lactic acid bacteria, are used to make yogurt, cheese, hot sauce, pickles, fermented sausages and dishes such as kimchee. A common effect of these fermentations is that the food product is less hospitable to other microorganisms, including pathogens and spoilage-causing microorganisms, thus extending the food's shelf-life.
Some cheese varieties also require molds to ripen and develop their characteristic flavors.
Lactic Acid Fermentation
During lactic acid fermentation, the electrons released during glycolysis are passed to pyruvic acid to form two molecules of lactic acid. Lactic acid fermentation is carried out by many bacteria, most notably by the lactic acid bacteria used in the production of yogurt, cheese, sauerkraut, and pickles. Some animal cells such as muscle cells can also use fermentation for a quick burst of energy.
Alcohol Fermentation
Alcohol fermentation also begins with glycolysis to produce two molecules of pyruvic acid, two molecules of ATP, and four electrons. Each pyruvic acid is modified to acetaldehyde and CO2. Two molecules of ethyl alcohol are formed when each acetaldehyde molecule accepts two electrons. Alcohol fermentation is carried out by many bacteria and yeasts .
83 |
)Production of beer and other alcoholic beverages |
Brewing is the production of beer through steeping a starch source (commonly cereal grains) in water and then fermenting with yeast. Brewing hastaken place since around the 6th millennium BC, and archaeological evidence suggests that this technique was used in ancient Egypt. Descriptions of various beer recipes can be found in Sumerian writings, some of the oldest known writing of any sort.[1][2][3] Brewing is done in a brewery by a brewer, and the brewing industry is part of most western economies.
The basic ingredients of beer are water; a starch source, such as malted barley, which is able to be fermented (converted into alcohol); a brewer's yeastto produce the fermentation; and a flavouring, such as hops. A secondary starch source (an adjunct) may be used, such as maize (corn), rice or sugar. There are several steps in the brewing process, which include malting, milling, mashing, lautering, boiling, fermenting, conditioning, filtering, andpackaging. There are three main fermentation methods, warm, cool and wild or spontaneous. Fermentation may take place in open or closed vessels. There may be a secondary fermentation that can take place in the brewery, in the cask, or in the bottle.
Brewing specifically includes the process of steeping, such as with making tea, sake, and soy sauce.
Wine production
