22. 180/ It is known that human immunodeficiency virus belongs to Retroviridae family. What basic feature characterizes this family?

A. Simple viruses, which affect only human beings.

B. Contain RNA of negative polarity.

C. Presence of reverse transcriptase enzyme.

D.Nucleic acid does not integrate into the genome of the host.

E. DNA-containing viruses which cause chronic infections.

22. “B” A young man appealed to anonymous cabinet for diagnosis of HIV-infection first time. Which test can be used for diagnosis of HIV-infection at this patient?

    1. Immunoenzyme assay (ELISA)

    2. Immunoblotting test

    3. Radioimmune assay

    4. Immune-electron microscopy

    5. Immunofluorescence test

23. There is a laboratory for diagnosis of HIV-infection in the large dental department. What diagnostic kits should be such laboratory equipped with?

    1. Cell cultures for virus isolation

    2. Test-systems for antibody detection with ELISA

    3. Special nutrient media kits

    4. Standard diagnostic sera

24. A patient with reccurent and resistant to therapy oral candidasis appealed to dentist for medical aid. It was revealed he had been having fever and weight loss for long time before. What investigation should be made in this case?

    1. Immunological and serological testing for HIV-infection

    2. Bacteriological tests for disbiosis

    3. Isolate pure culture of causative agent and detect its sensitivity to antibiotics

    4. Check the state of humoral immunity

    5. Detect the amount of specific antibody to Candida

25. For diagnostics of HIV-infection blood serum is tested by revealing of special antigen with ELISA. What enzyme-linked antibody is used in this case?

    1. Against protein gp41

    2. Against protein p24

    3. Against protein gp120

    4. Against protein p17

    5. Against human immunoglobulin

22. “B” Which method of donor blood checking has to be used to ensure absence of HIV in it?

    1. Revealing of viral nucleic acid

    2. Revealing of virus by infection of lymphocyte culture

    3. Revealing of IgM antibody

    4. Electron microscopy of T4 lymphocytes

    5. Revealing of causative agent antigens

23. 187/ During immunoblot analysis protein gp120 has been revealed in blood serum. What infection can be diagnosed by this antigen detecting?

A. Virus hepatitis B.

B.HIV-infection.

C.Tuberculosis.

D. Syphilis.

E.Poliomyelitis.

22. “B” Medical meeting was hold after revealing some cases of HIV infection among patients of dental clinics and rooms at which epidemiologists from Center of HIV-infection prophylaxis spoke about recommendation. Which measures of prevention could be recommended for dentists to prevent professional infection?

    1. To work in individual protectors (gloves, glasses)

    2. To wash hands after examination of each patient

    3. To use new sterile tool set for each patient

    4. To require a medical certificate from each patient

    5. To disinfect a room with quartz lamp

23. “B” HIV-infected woman gave a birth for baby in probationary department of maternity hospital. Which from the listed diagnostics methods of HIV-infection is the most reasonable for this baby?

    1. Polymerase chain reaction (PCR)

    2. ELISA

    3. Neutralization test

    4. Complement fixation test

    5. Radioimmune assay

24. “B” Which stage of HIV-infection is person a source of infection at?

    1. At all stages of infection

    2. Acute HIV-infection

    3. Chronic (latent) HIV-infection

    4. Persistent generalized HIV-infection

    5. AIDS-associated complex

“07” After examining of the patient with aphthous stomatitis with corresponding candidiasis a stomatologist has decided to exclude HIV-infection. Which assay is helpful for clearing of this case and for primary diagnosis?

1. Phase-contrast microscopy

2. Gel precipitation test

3. Immunoenzyme assay

4. Inhibition of hemagglutination test

5. Hemagglutination test

22. “B” A patient, which applied to hospital for results of ELISA, was given with primary diagnosis of HIV-infection. Which serological test should be used for confirmation of diagnosis?

    1. Immunoblotting

    2. Agglutination test

    3. Complement fixation test

    4. Thermoprecipitation test

    5. Immunofluorescence test

23. 165/ During checking the donors' blood at a hemotransfusion station antibodies to human immunodeficiency virus have been revealed in the serum. What method is recommended for the confirmation of HIV-infection diagnosis?

A. Complement fixation test.

B. Electron microscopy.

C. Western blotting (immunoblotting).

D. Immunofluorescence test.

E. Radioimmunoassay

1/ A patient with unknown fever, immunodeficiency state, gastrointestinal disorders nd nervous system failure has been given with diagnosis “AIDS”. Which methods of laboratory diagnostics have to be used for confirmation of diagnosis?

1. Polymerase chain reaction, immunoblot, immunoenzyme assay

2. Complement fixation test

3. Agglutination test

4. Hemadsoption test

5. Hemagglutination test

“2013” A patient consulted an immunologist about diarrhea, weight loss within several months, low-grade fever, and enlarged lymph nodes. The doctor suspected HIV infection. What immunocompetent cells must be studied first of all?

1. Helper T-lymphocytes

2. B-lymphocytes

3. Plasma cells

4. Monocytes

5. Suppressor T-lymphocytes

167/ A patient has been prescribed a combination of preparations, which inhibit HIV reproduction in the specialized clinic. To what group do the preparations, which are obligatory used in complex antiviral treatment, belong?

A. Interleukin.

B. Broad spectrum antibiotics.

C. Nucleosides analogs.

D. Nystatin.

E. Biseptol.

166/ HIV-infected patient is examined time to time to estimate the signs of process activation. Name the most important sign that arise at AIDS development.

1. Number of neutrophiles is getting less

2. Kaposi’s sarcoma. T-helpers number is less then critical data

3. T-suppressor number is getting higher

4. T-helpers number is less then critical data

5. Revealing of antibody to gp41

“04” during examination of patient with AIDS two positive results of ELISA were obtained. Which test must be used for exception of false-positive results for ELISA?

1. Luminescent microscopy

2. Molecular hybridization

3. Immunofluorescent test

4. *Immunoblot

5. Radioimmune assay

“04” A patient 29 y.o.was admitted to hospital with pneumonia. At microbiological assay of sputum Pneumocystus carinii was revealed. Which infectious disease only does this etiological form of pneumonia develop at?