482. It is necessary to use direct variant of ift for express diagnostics of anthrax. What ingredient must be used to perform the reaction?
А. Luminescent serum.
В. Immune antibacterial serum.
С. Immune antitoxic serum.
D. Toxoid.
Е. Protective antigen.
483. Numerous deaths of rats were registered in some of the farms of village K. It was supposed that the causative agent might be that of plague. What serological reaction had to be used for express identification of the causative agent?
А. Precipitation test.
В. Agglutination test.
С. Passive haemagglutination test.
D. Complement fixation test.
Е. Neutralization test.
484. Reconnaissance data certifies that the arsenal of the army of country Z. contains bacteriological weapon (agent of plague). What preparation should the soldiers of their neighbour, state N., be introduced with if country N. is going to wage a war on the territory of country Z.?
А. Vaccine EV76.
В. Vaccine SEI23.
С. Vaccine BCG.
D. Vaccine DTP.
Е. Plague serum.
485. A man that had received an envelope with unknown powder applied to the infectious department. The man was admitted to the hospital to a separate box; powder from the envelope was sent to the lab to identify the agent of anthrax. What method of investigation will be the most effective in this case?
А. Immunofluorescent test.
В. Complement fixation test.
С. Precipitation test in gel.
D. Isolation of pure culture.
Е. Biotest on mice.
486. A veterinary was admitted to the hospital with the suspicion of brucellosis. On the basis of what serological test can the diagnosis be confirmed or denied?
А. Wright’s test.
В. Widal test.
С. Ascoli test.
D. Veigle test.
Е. Wassermann test.
487. To confirm the diagnosis of tularemia in a hunter hospitalized on the 5th day of illness it is necessary to perform:
А. Allergy test.
В. AT.
С. PHAT.
D. CFT.
Е. IFT.
488. What test should be administered to confirm the diagnosis of brucellosis?
А. Serological (Wright’s test and Huddleson test).
В. Isolation of the agent in the blood.
С. Isolation of the agent in the urine.
D. Isolation of the agent in the stools.
Е. Isolation of the agent in the cerebrospinal fluid.
489. Dairy maids are checked up with skin allergic Burnet’s test that is used to reveal hypersentivity to:
А. Brucellin.
В. Tuberculin.
С. Alt-tuberculin.
D. Tularin.
E. Anthraxin.
490. Pure culture of the causative agent of plague was plated on Petri dish. After cultivation on the growth medium colonies of 1-1.5 mm in diameter were created without growing of the culture. How can it be interpreted?
А. Presence of bacteriophage in the culture.
В. Insufficient quantity of the germ in the material for inoculation.
С. Low quality of the medium.
D. Insufficient amount of vitamins in the medium.
Е. Aging of the culture.
491. What method of laboratory diagnostics is frequently used to confirm the diagnosis of brucellosis?
А. Serological (Wright’s reaction).
В. Serological (Huddleson test).
С. Serological (PHAT).
D. Express-method (IFT).
Е. Serological (Coomb’s reaction for identification of incomplete antibodies).
492. Diagnosis of plague seems to be confirmed by executed reaction of immunofluorescence. Characterize the results of microscopy obtained at that reaction.
А. Small oval rods with bright green luminescence.
В. Small cocci of pink colour.
С. Large bacilli with cut endings of violet colour.
D. Small bacilli with oval endings of pink colour.
Е. Curved red bacilli located angular to each other.
493. A vet of the farm was admitted to hospital complaining of pain in joints, fever, and sweating at night. Since he’d been ill for about a month, the doctor suspected brucellosis. What material obtained from the patient must be investigated in the microbiological laboratory?
А. Blood serum.
В. Cerebrospinal fluid.
С. Vomiting mass.
D. Urine.
Е. Feaces.
494. Skin Burnet’s test is positive in a patient diagnosed with brucellosis. What factor of the immune system plays the basic part in the development of inflammatory reaction in the place of brucellin introduction?
А. Sensibilizated T-lymphocytes.
В. IgA.
С. IgE.
D. IgG.
Е. IgD.
495. The material (extract from animal raw) from the district where cases of anthrax had been registered was sent to the laboratory. What serological reaction should be performed to reveal antigens of bacillus anthracis in the material?
А. Ring test − Ascoli test.
В. Complement fixation test.
С. Passive hemagglutination.
D. Radioimmune assay.
Е. Precipitation test in gel.
496. A patient with carbuncle on the face was admitted to the hospital. Results of examination of the carbuncle: it is not dense, painless, subcutaneous fat is edematous, there is black eschar in the center of the carbuncle; periferal vesicular rash. Bacteriological examination revealed immobile streptobacilli creating capsules. What microorganisms are the causative agents of the disease?
А. Bacillus anthracis.
В. Staptylococcus aureus.
С. Bacillus anthracoides.
D. Bacillus subtilis.
Е. Bacillus megaterium.
497. A patient complaining of symptoms typical for tularemia is admitted to the hospital. What method is suggestive in this case for express diagnostics of the disease?
А. Skin-allergic test.
В. Biological.
С. Serological (agglutination reaction).
D. Bacteriological (isolation of pure cultures).
Е. Microscopic.
498. Microbiological laboratory has to investigate the material suspected to be contaminated with spores of bacillus anthracis. What diagnostic preparation is used for identification of the causative agent?
А. Luminescence antianthrax serum.
В. Antianthrax immunoglobulin.
С. Standard anthrax antigen.
D. Enzyme-signed immunoglobulin.
Е. Monoclonal antianthrax antibodies.
499. Investigating animals’ skin, bacteriologist performed reaction of thermal ring precipitation (Ascoli test). Fixation of immune serum with skin’s extract produced a whitish ring. What do the results of the reaction testify?
А. Presence of toxin of anaerobic infection.
В. Presence of brucellosis causative agent.
С. Presence of superficial antigen of escherichia.
D. Presence of antigen of virulence of salmonella.
E. Presence of anthrax antigen.
500. During the epidemic of grippe a dairymaid visited a doctor complaining of high temperature, weakness, loss of appetite, and pain in joints. She was treated for grippe within 10 days, but was initially diagnosed with brucellosis. What test is likely to confirm the diagnosis of brucellosis?
А. Wright’s test.
В. Huddleson’s test.
С. Immunofluorescence test.
D. Widal reaction.
E. Ouchterlony reaction.
501. A farmer rearing goats developed fever of unknown etiology. What species of bacteria is likely to cause the fever?
А. Brucella melitensis.
В. Clostridium novyi.
С. Treponema pallidum.
D. Histoplasma capculatum.
Е. Mycobacterium tuberculosis.
502. Biological test of smear prints obtained form the animal revealed streptobacilli surrounded by a capsule. What diagnosis based on the data of the test is suggestive in this case?
А. Tularemia.
В. Anthrax.
С. Plague.
D. Brucellosis.
E. Croupous pneumonia.
503. A 28-year-old patient, a worker of a slaughter-house, is admitted to the hospital on the 4th day of illness with the following symptoms: edema of the right hand, ulcer covered with the dark crust, and hyperemia. Margins of ulcer are noticible with vesicular rash. Microscopy of the pathologic material stained by Gram and Drobotko method revealed immobile Gram-positive rods with cut ending located in pairs or chains and surrounded by microcapsule. What species of bacteria was identified by microscopy?
А. Clostridium histolyticum.
В. Bacillus anthracis.
С. Bacillus megaterium.
D. Bacillus mycoides.
Е. Clostridium perfringens.
504. A 20-year-old patient, a worker of rodent control of sanitary station, presented to the infectious department complaining of severe chills, high temperature (39 °C), headache, and inflammation of groin lymphatic node, which complicated the motion of the leg while walking. Purulent contents of the lymphatic node and blood smear were investigated by microbiologist. Colorless microcolonies with rough outlines ("broken glass") appeared on culture medium 8 hours later. Within 18-24 hours of cultivation, colonies with turbid-white centre, surrounded by rough border ("lacy shawls") grew on nutrient medium. On MPA microorganisms created tender pellicle with threads similar to stalactites. What microorganism is identified in the material?
А. Clostridium tetani.
В. Chlamydia psittaci.
С. Bacillus anthracis.
D. Yersinia pestis.
Е. Trichophyton verrucosum.
505. The following test to confirm an initial diagnosis was performed by bacteriologist: 1) inoculation of pathological material on liver and sugar broths; 2) estimation of hypersensitivity of isolates to aniline dye-stuff; 3) serological reactions of Wright and Huddleson; 4) Burnet’s skin allergic test. What is the diagnosis of the disease?
А. Tularemia.
В. Typhoid fever.
С. Salmonellosis.
D. Q-fever.
Е. Brucellosis.
506. Investigation of brucellosis episode among the workers of a farm was performed by express method of Huddleson reaction with 5 positive results obtained. What test of the patients’ blood serum is likely to confirm the diagnosis?
А. Reaction of Middlebrook – Dubo.
В. Schick test.
С. Reaction of Voges-Proskauer.
D. Kahn’s reaction.
Е. Wright’s test.
507. A 40-year-old patient with acute fever of unknown etiology is administered serological blood test on the eighth day of the disease. Reaction of agglutination with various diagnostics testifies that Widal test is positive with serum titer 1:100, Wright test is positive with serum titer 1:400. What diagnosis is likely to be made on the basis of the results of serological investigation?
А. Leptospirosis.
В. Paratyphoid A.
С. Typhoid fever.
D. Brucellosis.
Е. Paratyphoid B.
508. A patient is initially diagnosed with acute pneumonia. Microscopy of the patient’s sputum revealed chaotically arranged ovoid microorganisms, 2.0 µm long and bipolar stained. What seems to be the reliable diagnosis of the disease?
А. Staphylococcal pneumonia.
В. Pneumococcal pneumonia.
С. Diphtheria.
D. Klebsiella pneumonia.
Е. Pneumonic plague.
509. A 42-year-old butcher visited a doctor complaining of swelling, redness, and acute pain in dorsal surface of the wrist. Examination of the wrist revealed conoid red infiltrate with marked edema. What is the initial diagnosis of the disease?
А. Abscess.
В. Plague.
С. Furunculosis.
D. Phlegmon of hand.
Е. Anthrax.
510. It is necessary to determine LD50 of vaccine strain of Bacillus anthracis to estimate its residual virulence. What method is the most suitable?
А. Quantitative registration of microorganisms at cultivation on nutrient medium.
В. Revealing enzymes of pathogenicity.
C. Studying biochemical activity.
D. Infecting of laboratory animals.
E. Determination of toxin serotype produced by microorganism.
511. Sputum of the patient with severe acute pneumonia was bacterioscopically investigated. Gram staining revealed Gram-positive streptobacilli with cut endings. What species of bacteria was identified by bacteriologist?
А. Bacillus anthracis.
В. Yersinia pestis.
С. Streptococcus pneumoniae.
D. Klebsiella pneumoniae.
Е. Bacillus anthracoides.
512. Epizootic case of plague among gophers was recorded in one of the rural areas after a 20 year period of normal epidemic situation. The residents of the region had been immunized 5 years before. All the residents of the age of 7 and older were vaccinated. What can be suggested for specific prophylaxis in the region?
А. To vaccinate medical personnel and workers of rodent control.
В. To vaccinate persons who are older than 12 years.
С. To vaccinate persons of 12- 15 years old.
D. Not to vaccinate inhabitants.
Е. To vaccinate all the inhabitants.
513. What method of diagnostics is suggested for identification of Bacillus anthracis while investigating specimen of animals’ skin?
А. Express-method (Ascoli test).
В. Bacterioscopic method (microscopy of preparation stained by Burri-Gins).
С. Bacterioscopic method (microscopy of preparation stained by Ozheshko).
D. Bacteriological method.
Е. Serological method.
514. A group of 20 students of veterinary college are appointed for seasonal work to the district with unfavourable conditions (because of tularemia). Some of the students worked there last summer and had been vaccinated against tularemia before. What should be done to provide specific prophylaxis?
А. To vaccinate a group of students except the students vaccinated last year.
В. To vaccinate all the students.
С. To vaccinate a group of students who worked in that district a year before.
D. Not to vaccinate students.
Е. To deliver a lecture about prophylaxis of tularemia.
515. A young boy came in contact with a sick patient diagnosed with pneumonic plague. What remedy will be prescribed for express nonspecific prophylaxis of plague?
А. Antibiotics.
В. Plague immunoglobulin.
С. Plague bacteriophage.
D. Attenuated (live) vaccine.
Е. Toxoid.
516. Punctate of the lymphatic node was delivered to the bacteriological laboratory. Bacteriologist inoculated material on nutrient agar. It was cultivated in thermostat at the temperature of 37 °C and of 28 °C. The most intense growth of culture was observed at t = 28 ºC. What microorganism is likely to be identified by bacteriologist?
А. Yersinia pestis.
В. Staphylococcus aureus.
С. Francisella tularensis.
D. Brucella abortus.
Е. Treponema pallidum.
517. A bioterrorist sent powder in envelopes which might contain causative agent of anthrax. A parcel may be dangerous for a long time because the causative agent of anthrax:
А. Forms spores.
В. Has flagella.
С. Forms protein capsule.
D. Forms polysaccharide capsule.
Е. Belongs to actinomycetes.
518. A patient was admitted to hospital with a severe case of pneumonia. Rough colonies with uneven borders grew from the patient’s sputum inoculated on MPA. Bacterioscopy revealed large Gram-positive bacilli surrounded by capsule and located in chains. What microorganism is characterized by such properties?
А. Bacillus anthracis.
В. Streptococcus pneumoniae.
С. Klebsiella pneumoniae.
D. Mycobacterium tuberculosis.
Е. Bordetella pertussis.
519. Microscopy of ulcer covered by dark eschar and surrounded by edema and hyperemia, revealed Gram positive bacilli with cut endings. Microorganisms were arranged in capsules separately or in chains. What is the causative agent of the disease?
А. Bacillus anthracis.
В. Mycobacterium tuberculosis.
С. Clostridium perfringens.
D. Corynebacterium diphtheriae.
Е. Yersinia pestis.
520. A patient diagnosed with brucellosis was admitted to the infectious department. A physician suspected bacteremia at that period of illness. What material was sent to the laboratory for investigation?
А. Blood.
В. Urine.
С. Feaces.
D. Cerebrospinal fluid.
Е. Sputum.
521. A geologist who has recently come back from the expedition is complaining of symptoms typical for plague. On examination, symptoms of pneumonia are noticible. What method of staining will allow bacteriologist to reveal typical for plague bipolar staining of bacilli?
А. Leoffler’s method (methylene blue).
В. Pfeiffer’s fuchsine.
С. Ziehl’s fuchsine.
D. Gram stain.
Е. Romanovsky-Giema’s stain.
522. Microbiological investigation of eschar of cattle breeder suspected of anthrax revealed Gram-positive bacilli surrounded by a capsule. Cultivated on the medium, they created colonies with uneven, fibrous, curly border (Lion’s mane). What prophylactic remedy should be prescribed for relatives who were in contact with the patient and sick animals?
А. Antianthrax γ (gamma) - globulin.
В. Vaccine SEI.
С. Vaccine EV76.
D. Antitetanus serum.
Е. Sextatoxoid.
523. Bacteriologist of the laboratory of particular dangerous infections used biological method to investigate the material. What properties of the causative agent are unlikely to be studied through this method?
А. Phagolysis.
В. Biological.
С. Morphological.
D. Tinctorial.
Е. Antigenic.
524. Ovoid-shaped and bipolar stained Gram-negative bacterium was isolated from the corpse of rodents. The causative agent of what disease was identified by bacteriologist?
А. Causative agent of plague.
В. Causative agent of tuberculosis.
С. Causative agent of syphilis.
D. Staphylococcus.
Е. Streptococcus.
525. Large Gram-positive bacilli (5-10 × 1.5-2 μm) located in chain and forming a capsule were obtained from the corpse of a horse. What microorganism is it?
А. Causative agent of anthrax.
В. Causative agent of foot and mouth disease (aphthous fever).
С. Streptococcus.
D. Causative agent of hepatitis A.
Е. Causative agent of tetanus.
526. A worker of meat processing factory developed arthritis accompanied by inflammation of peripheral lymphatic nodes, pain, high temperature, and symptoms of allergy. The causative agent was not identified during laboratory investigation, but skin allergic Burnet’s test appeared to be positive. What microorganism could have caused the disease?
А. Causative agent of brucellosis.
В. Causative agent of tuberculosis.
С. Causative agent of anthrax.
D. Causative agent of salmonellosis.
Е. Causative agent of diphtheria.
527. Bacteriologist titred blood serum of the patient by physiologic solution 1:100, 1:200, 1:400, 1:800 and added brucellosis diagnosticum. What reaction was performed by bacteriologist for serological investigation of blood serum?
А. Wright’s test.
В. Passive hemagglutination test.
С. Precipitation test.
D. Gruber’s agglutination test.
Е. Passive indirect hemagglutination test.
528. Cases of anthrax were registered in a settlement. Health care workers began specific prophylaxis of anthrax among the residents of the area according to epidemiological indications. What preparation was used?
А. Attenuated (live) vaccine.
В. Inactivated vaccine.
С. Chemical vaccine.
D. Engineered vaccine.
Е. Toxoid.
529. What causative agent may be transmitted through a sting of a flea?
А. Causative agent of plague.
В. Causative agent of leptospirosis.
С. Causative agent of endemic typhus.
D. Causative agent of brucellosis.
Е. Causative agent of anthrax.
530. It is necessary to vaccinate a man who is going on an official journey to the region endemic on tularemia. What does the vaccine for prophylaxis contain?
А. Attenuated (live) strain of Francisella tularensis.
В. Francisella tularensis inactivated by formalin.
С. Endotoxin inactivated by formalin.
D. Attenuated (live) strain of Yersinia pestis.
Е. Mouse toxin inactivated by formalin.
531. A man walked across the locality where epizootic plague among rodents was observed. What could the man be infected by?
А. Flea.
В. Louse.
С. Fly.
D. Mosquito.
Е. Tick.
532. A patient with infectious disease responds positively to skin allergic Burnet's test. What diagnosis is the test likely to comfirm?
A. Q-fever.
B. Tularemia.
C. Typhoid fever.
D. Salmonellosis.
E. Brucellosis.
533. Investigating the stuff of animals (cutis, wool) for the presence of causative agent of anthrax, bacteriologist made soluble thermostable antigen in water salt extract. What reaction was used by bacteriologist?
A. Neutralization test.
B. Precipitation test in gel.
C. Agglutination reaction.
D. Passive hemagglutination test.
E. Ring test (Ascoli test).
534. Bacteriologist performed immune fluorescent test to indicate the causative agent of plague in the preparation made from enlarged lymphatic node (the preparation was processed by luminescent plague serum). Luminescent microscopy revealed shining bacteria. Bacteriologist related the results of the test with the presence of causative agent of plague in the preparation. How can luminescence of Yersinia pestis be interpreted?
А. Plague antibody marked by luminescent bound with antigen on the surface of plague causative agent producing shiny surface.
В. Antigen-antibody reaction took place on the surface of bacteria.
С. Luminescence pigmented bacteria in the preparation.
D. Yersinia pestis is autoluminescent.
E. Complex antigen-antibody on the surface of germ fixed the complement.
535. A man who took care of animals, died because of the clinical signs of sepsis. Carbuncle with serous hemorrhagic inflammation and local lymphadenitis was found on the right upper limb at autopsy. What disease are these signs typical for?
А. Anthrax.
В. Tularemia.
С. Brucellosis.
D. Plague.
E
. Tuberculosis.