Task 2. Reflex arc analysis.

To prepare spinal frog and to hang it with inferior jaw on a stand. To put one of her legs in 0,5% of sulfuric acid.

Convince in reflex existence. To perform round skin incision below knee jerk and to release the leg from its skin. To irritate this leg tibia again. To observe the reaction.

To cut other posterior (inferior) leg hip skin of the same frog and to prepare sciatic nerve over the distance of 1,5-2,0 cm. To bring the thread under the nerve but not to tie it. To call flexion reflex by means of taking this leg fingers ends into acid. Then to tighten the nerve carefully by the thread and put the cotton wool washing into novocain solution under it for excitement transduction blocking into sciatic nerve fibers. To check the reflex existence.

To check the reflexes existence on superior legs.

To destroy the spine and to observe all reflexes disappearance.

On the base of investigations performed make the conclusion about reflex arc structure. Designate its links.

Task 3. Gustatory receptors functional mobility determining (before and after eating).

To be convinced of eating leading to demobilization i.e. actively functioning gustatory receptors number decreasing.

The work must be performed on an empty stomach or at least after 3-4 hours after eating. The experienced person tongue must be dried with filter paper. Gustatory stimulus (sugar solution: 8 grams of sugar for 20 ml of dist water) must be taken on separate tongue fungiform papillas with pipette. 4 papillas becomes differentiated at this that give sweet taste sensation. It is one probe. Students must perform 5 probes with intervals 1-2 minutes between them. One must rinse mouth out after every probe. You should investigate the same papillas. The gustatory sensation appearance is marked in protocol with sign “+”, the disappearance- “-“. You should count positive answers common number and express mobilization level in per cents. The investigations must be repeated after eating (sweet tea glass with white bread).

5. Literature recommended:

1. Lecture course.

2. Mistchenko V.P., Tkachenko E.V. Methodical instructions for dental students (short lecture course).-Poltava, 2005.-P. 24-25.

3. Mistchenko V.P., Tkachenko E.V. Methodical instructions for medical students (short lecture course).-Poltava, 2005.-P.11-12.

4. Mistchenko V.P., Tkachenko E.V. Methodical instructions on Normal Physiology on practical classes for dental and medical students.-Poltava, 2005.-P.26-34.

5.Ganong W.F. Review of Medical Physiology.-21^st ed.-2003.-Section II.

6. Kapit W., Macey R.I., Meisami E. The Physiology Colouring Book: Harpers Collins Publishers, 1987.-P. 17, 83-86.

7. Bullock J., Boyle III J., Wang M.B. Physiology.-1991.- P.

8. Guyton – Ganong – Chatterjee. Concise Physiology /Ed. By Dr Raja Shahzad Gull: M.B.B.S., F.C.P.S., King Edward Medical College.-Lahore, 1998 (1^st Edition).-P. 261-263..

9. Guyton A.C. Textbook of Medical Physiology.-NY, 1992.-P. 572-577.

6.Materials for self-control:

A. Control questions.

1. The term “reflex”.

2. Reflex receptor zone.

3. Reflex reflectory arc and its structure.

4. Reflex time.

5. Reflexes classification.

6. Reflectory arcs types.

7. Receptors distingushing features.

8. Receptor and generator potential.

9. Receptors classification.