- •General microbiology (continued from spring semester)
- •The Scheme of Complement Fixation Test
- •In Enteric Escherichiosis (infant’s diarrhea)
- •The Scheme of Flocculation Test
- •Special microbiology section 1: special bacteriology (Enteric bacterial pathogens)
- •The Protocol of the Blood Culture Bacteriological Examination
- •In Suspected Enteric Fever
- •The Protocol of the Bacteriological Examination of Vomit Specimens
- •In Suspected Salmonellosis
- •The Scheme of Bacteriological Examination of Stool Specimens
- •The Scheme of Bacteriological Examination of Stool Specimens in Shigellosis
- •The Kaufmann-White’s Scheme of Salmonellae Classification
- •Section 2: special bacteriology
- •The Most Common Pathogens of Streptococcal Human Diseases
- •The Scheme of iha for Botulinum Toxin Identification
- •Determination of Average Concentration of Bacteria in Urine Sample
- •* Zoonoses
- •The Scheme of the Huddleson’s Test
- •The Scheme of the Wright’s Test
- •The Scheme of the Ascoli’s Test (the Precipitation Test for Diagnosis of Anthrax)
- •* Special virology
- •Identification of viruses. Serological tests used for making diagnosis of viral diseases. Viruses - the causative agents of respiratory tract diseases. Rabies virus. Smallpox virus
- •The Scheme of vha-test for Virus Indication in the Allantoic Fluid
- •The Results of the hai Test for the Serological Examination
- •Cpe of polioviruses (destruction of monolayer)
- •The Scheme of elisa for hBs antigen Detection in a Patient’s Serum
The Results of the hai Test for the Serological Examination
of the Patient’s Paired Sera in Influenza
RESULTS for: |
Serum dilutions |
Controls of: |
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1:20 |
1:40 |
1:80 |
1:160 |
1:320 |
1:640 |
v.A1c. |
v.A3c. |
e.c. |
a.s.c. |
c.s.c. |
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With diagnosticum A1 (H1N1) |
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Acute serum |
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Convalescent serum |
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With diagnosticum A3 (H3N2) |
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Acute serum |
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Convalescent serum |
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CONCLUSION: The HAI test is positive with diagnosticum _________. The antibody titer in acute serum is ________. The antibody titer in convalescent serum is ______. (The four-fold titer rising is of diagnostic value). |
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* NOTE: v.A1c. -virus A1 control; v.A3c. -virus A3 control; e.c. – control of erythrocytes; a.s.c. –acute serum control; c.s.c. – convalescent serum control
**NOTE: enter the results of test: «+» if hemagglutination occurs (HAI is negative);
«-» if there is no hemagglutination (HAI is positive).
Table 30
The Protocol of the Microbiological Diagnosis of Influenza
Date |
Methods and manipulations |
Results and conclusions |
First day |
Inoculation of chicken embryos with patient’s respiratory secretions
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After 48 h |
Indication: VHA-test with allantoic fluid from the infected embryo Identification: CF- test for serotyping and HAI- test for subtyping |
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After 2 - 3 weeks |
Serological examination of the patient’s paired sera in HAI-test |
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CONCLUSION: The infection (in the patient examined) was caused by Influenza virus subtype____. |
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Picture 7. The Virus Neutralization Test in the Cell Culture (for Adenovirus Identification)
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adenovirus tested + antiserum 3 |
adenovirus tested + antiserum 4 |
adenovirus tested + antiserum 7 |
Controls of: |
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cell culture |
virus |
antiserum 3 |
antiserum 4 |
antiserum 7 |
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CONCLUSION:
LESSON 13 Date:
ENTEROVIRUSES. VIRAL HEPATITIS A, B, C, D and E.
Picture 8. The Virus Neutralization Test in the Cell Culture (for Poliovirus Identification)
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poliovirus tested + antiserum I |
poliovirus tested + antiserum II |
poliovirus tested + antiserum III |
Controls of: |
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cell culture |
virus |
antiserum I |
antiserum II |
antiserum III |
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CONCLUSION:
Picture 9. The Virus Neutralization Test in the Cell Culture (for serological examination of paired patient’s sera in poliomyelitis)
Acute serum |
Convalescent serum |
Controls |
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1:50 |
1:100 |
1:200 |
1:400 |
1:800 |
1:50 |
1:100 |
1:200 |
1:400 |
1:800 |
cell culture |
virus |
acute serum |
convalesce. serum |
CONCLUSION: