- •General microbiology (continued from spring semester)
- •The Scheme of Complement Fixation Test
- •In Enteric Escherichiosis (infant’s diarrhea)
- •The Scheme of Flocculation Test
- •Special microbiology section 1: special bacteriology (Enteric bacterial pathogens)
- •The Protocol of the Blood Culture Bacteriological Examination
- •In Suspected Enteric Fever
- •The Protocol of the Bacteriological Examination of Vomit Specimens
- •In Suspected Salmonellosis
- •The Scheme of Bacteriological Examination of Stool Specimens
- •The Scheme of Bacteriological Examination of Stool Specimens in Shigellosis
- •The Kaufmann-White’s Scheme of Salmonellae Classification
- •Section 2: special bacteriology
- •The Most Common Pathogens of Streptococcal Human Diseases
- •The Scheme of iha for Botulinum Toxin Identification
- •Determination of Average Concentration of Bacteria in Urine Sample
- •* Zoonoses
- •The Scheme of the Huddleson’s Test
- •The Scheme of the Wright’s Test
- •The Scheme of the Ascoli’s Test (the Precipitation Test for Diagnosis of Anthrax)
- •* Special virology
- •Identification of viruses. Serological tests used for making diagnosis of viral diseases. Viruses - the causative agents of respiratory tract diseases. Rabies virus. Smallpox virus
- •The Scheme of vha-test for Virus Indication in the Allantoic Fluid
- •The Results of the hai Test for the Serological Examination
- •Cpe of polioviruses (destruction of monolayer)
- •The Scheme of elisa for hBs antigen Detection in a Patient’s Serum
Cpe of polioviruses (destruction of monolayer)
Table 31
The Scheme of elisa for hBs antigen Detection in a Patient’s Serum
Ingredients |
Test |
«Positive control» |
«Negative control» |
|||||
A1 A2 |
B1 B2 |
C1 C2 |
D1 D2 |
E1 E2 |
F1 F2 |
G1 G2 |
H1 H2 |
|
1. Buffer A (ml) |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
Incubation at room temperature for 10 min. |
||||||||
2. Patient’s serum (=antigen tested) (ml) |
0.1 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
3. Standard HBs antigen 100 μg/ml (ml) |
-- |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 pour out |
-- |
4. Reagent «negative control» (ml) |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
0.1 |
Incubation at 37oC for 20 min. |
||||||||
5. Conjugate (ml) |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
Incubation at 37oC for 20 min. |
||||||||
6. Substrate (ml) |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
Incubation at room temperature for 20 min. |
||||||||
7. 50% sulfuric acid (ml) |
0.05 |
0.05 |
0.05 |
0.05 |
0.05 |
0.05 |
0.05 |
0.05 |
RESULTS: (color/no color) |
|
|
|
|
|
|
|
|
CONCLUSION: the ELISA is ________________(positive/negative). The result means, that HBs Ag is (not) detected in the patient’s serum. The concentration of HBs Ag is _______ μg/ml.
|
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