The Scheme of iha for Botulinum Toxin Identification

Ingredients

Dilutions of tested specimens

1:10

1:20

1:40

1:80

1:160

1:320

1:640

control

Isotonic solution, ml

0.5

0.5

0.5

0.5

0.5

0.5

0.5

0.5

Tested material (1:5), ml

0.5













pour out

--

Erythrocytic antibody-containing diagnosticum, ml

0.5

0.5

0.5

0.5

0.5

0.5

0.5

0.5

RESULTS:

Polyvalent antibody-containing diagnosticum ABE

Monovalent antibody-containing diagnosticum A

Monovalent antibody-containing diagnosticum B

Monovalent antibody-containing diagnosticum E

CONCLUSION: the botulinum toxin type _____ is detected and identified in patient’s gastric lavage waters

Examination day (step)

Manipulations

Results and conclusions

1

Inoculation of MPA with urine tested by using Gold’s technique

2

1. Microscopic examination of colonies (Gram stain)

2. Subculture on the slant

3

1. Antibiogram

• ______________- ___ mm ( )

• ______________- ___ mm ( )

• ______________- ___ mm ( )

• ______________- ___ mm ( )

• ______________- ___ mm ( )

CONCLUSION: The tested urine sample is _____________ (sterile/not sterile).

The isolated microbe is identified as ______________________.

The average concentration of isolated microbe in the tested urine sample is ____CFU/ml, that confirms/does not confirm its etiologic role in the development of symptoms of pyelonephritis.

It is mostly sensitive to_________________________________

and resistant to_________________________________________.