* Special virology
LESSON 12 Date:
Identification of viruses. Serological tests used for making diagnosis of viral diseases. Viruses - the causative agents of respiratory tract diseases. Rabies virus. Smallpox virus
Table 26
The Scheme of vha-test for Virus Indication in the Allantoic Fluid
Ingredients |
Dilutions of tested virus-containing material |
Erythrocytes control |
||||
1:2 |
1:4 |
1:8 |
1:16 |
1:32 |
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Isotonic solution, ml |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
Allantoic fluid (virus-containing material), ml |
0.5 |
|
|
|
pour out |
-- |
1% suspension of human erythrocytes, ml |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
Shake carefully. Incubate at room temperature for 30 min. |
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RESULTS: |
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CONCLUSION: the VHA is positive/negative. The virus with hemagglutinins is detected/not detected in allantoic fluid. The virus titer is ______. |
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*NOTE: enter the results of test: «+» if hemagglutination occurs; «-» if there is no hemagglutination.
Table 27
The Complement Fixation Test for the Identification of the Influenza Virus Serotype
RESULTS for: |
Serum dilutions |
Controls* |
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1:20 |
1:40 |
1:80 |
1:160 |
1:320 |
1:640 |
h.s.c. |
c.c. |
v.c. |
s.Ac. |
s.Bc. |
|
Anti-serum against serotype A virus |
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Anti-serum against serotype B virus |
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CONCLUSION: the CF-test is positive with _________ antiserum. The virus tested is identified as influenza virus serotype ____(A or B). |
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* NOTE: h.s.c. -“hemolytic system” control; c.c. - complement control; v.c. – virus control;
s.Ac. -anti-serum A control; s.Bc. - anti-serum B control
**NOTE: enter the results of test: «+» if hemolysis occurs (CF is negative); «-» if there is no hemolysis (CF is positive).
Table 28
The Scheme of HAI Test for the Influenza Virus Subtype Identification
Ingredients |
Antiserum dilutions |
Controls of: |
|||||||
1:20 |
1:40 |
1:80 |
1:160 |
1:320 |
1:640 |
Virus |
Serum A1/A3 |
RBC |
|
Isotonic solution (ml) |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
1.0 |
1st row: Antiserum to virus A1 (H1N1) 1:10 (ml) |
0.5 |
|
|
|
|
0.5 pour out |
--- |
0.5 |
--- |
2nd row: Antiserum to virus A3 (H3N2) 1:10 (ml) |
0.5 |
|
|
|
|
0.5 pour out |
--- |
0.5 |
--- |
Virus tested 4AU (ml) |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
--- |
--- |
1 % erythrocyte suspension (ml) |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
Total volume in the well |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
RESULTS: |
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Antiserum to virus A1 (H1N1)* |
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Antiserum to virus A3 (H3N2)* |
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CONCLUSION: The HAI-test is ___________ (positive/negative) with antiserum__________. The unknown virus is identified as Influenzavirus subtype________. |
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*NOTE: enter the results of test: «+» if hemagglutination occurs (HAI is negative);
«-» if there is no hemagglutination (HAI is positive).
__CPE_of rabies virus__ _ CPE of Adenovirus _ Hemadsorption test
(cytoplasmic inclusion bodies) (intranuclear inclusion bodies)
Table 29