The Scheme of Bacteriological Examination of Stool Specimens in Shigellosis

Examination day (step)

Manipulations

Results and conclusions

The First

The Second

The Third

CONCLUSION: the isolated microbe is identified as __________________________ serogroup _______ that confirms the diagnosis of __________________.

Results for:

Serum dilutions

antigen control

1:10

1:20

1:40

1:80

1:160

1:320

1:640

Acute serum

Convalescent serum

CONCLUSION: The test with O-diagnosticum is ________________(positive/negative).

The antibody titer in acute serum is _____.

The antibody titer in convalescent serum is ______.

(The four-fold titer rising is of diagnostic value).

Kligler iron agar

Simmons’ citrate medium

Lysine-containing medium

• Non-inoculated culture media

• Salmonella sp.

• Shigella sp.

• Escherichia coli

Serogroup, serovar, species

O-antigen

H-antigen

Phase 1

Phase 2

Serogroup 2 (A)

• S. Paratyphi A

1, 2, 12

a

(1, 5)

Serogroup 4 (B)

• S. Paratyphi B

(S. Schottmuelleri)

1, 4(5), 12

b

1, 2

• S. Typhimurium

1, 4(5), 12

i

1, 2

Serogroup 6,7 (C)

• S. Choleraesuis

6, 7

( c )

1, 5

Serogroup 9 (D₁)

• S. Typhi

9, 12

d

-

• S. Enteritidis

1, 9, 12

g, m

(1, 7)

• S. Dublin

1, 9, 12

g, p

-

• S. Moscow

9, 12

g, q

-

Serogroup 3,10 (E₁)

• S. London

3, 10

l, v

1, 6

Features

B.pertussis

B.parapertussis

• Growth on MPA

-

+

• Growth on tyrosine-containing medium

-

+ (brown pigment)

• Time of exposure (in the incubator)

3 - 5 days

48 h.

• Brown pigmentation of a colonies

-

+

• Urease test

-

+

• Catalase test

-

+

• Agglutination by:

• B.pertussis-antiserum

• B.parapertussis-antiserum

+

-

-

+