The Scheme of the Wright’s Test
(the Tube Agglutination Test for Serological Diagnosis of Brucellosis)
Ingredients |
1 |
2 |
3 |
4 |
5 |
serum |
antigen |
control |
control |
||||||
Isotonic solution (ml) |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
Patient’s serum 1: 25 (ml) |
1.0 |
|
|
|
pour out |
1.0 |
-- |
The serum dilution |
1:50 |
1:100 |
1:200 |
1:400 |
1:800 |
1:25 |
-- |
Diagnosticum (ml) |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
-- |
0.5 |
Results: |
|
|
|
|
|
|
|
CONCLUSION: The agglutination test is ______________ (positive/negative). The titer of antibodies against Brucella diagnosticum is__________. The diagnosis of brucellosis is (is not) confirmed. |
|||||||
Table 24
The Distinguishing Features of Brucella sp.
Species |
Culturing conditions |
Bacteriostatic Effect of Aniline Dyes |
H2S production |
||
Fucshin 1:50000 |
Thyonin 1:25000 |
Pyronin 1:200000 |
|||
Brucella melitensis |
Aerobic |
+ (growth) |
-- (no growth) |
+ (growth) |
-- |
Brucella suis
|
Aerobic |
-- (no growth) |
+ (growth) |
-- (no growth) |
-- |
Brucella abortus |
5 – 10 % CO2 |
+ (growth) |
-- (no growth) |
+ (growth) |
+ |
Table 25
The Scheme of the Ascoli’s Test (the Precipitation Test for Diagnosis of Anthrax)
Ingredients |
1 |
controls |
|
2 |
3 |
||
Precipitating antiserum against heat-stable antigen of B.anthracis (ml) |
0.2 |
0.2 |
-- |
Antigen tested (ml) |
0.2 |
-- |
-- |
Standard heat-stabile antigen of B.anthracis (ml) |
-- |
0.2 |
0.2 |
Non-immune antiserum (normal antiserum) (ml) |
-- |
-- |
0.2 |
Results: |
|
|
|
CONCLUSION: The precipitation test is ______________ (positive/negative). The specimen tested contains (does not contain) antigens of B. anthracis. |
|||
__Yersinia pestis___ __Brucella abortus_
(pure culture) ___ __(pure culture)____
Crystal Violet Dye Gram Stain
__Francisella tularensis_ __Bacillus anthracis __
(pure culture) ___ ____(pure culture)____
Gram Stain Gram Stain