- •Contents:
- •Introduction
- •Structure, functions and methods of research of biological membranes The general structure of cell
- •Ultrastrucrure and function of function of biological membrane
- •Kinds of passive transport
- •Methods of research of structure and functions of biological membranes
- •Method of a dark field.
- •Phase - contrast method.
- •Electronic microscopy.
- •Method of diffraction of X-rays radiation.
- •Ultramicroscopy.
- •Luminescent methods of research of membranes.
- •Method of ultrachemistry.
- •Nuclear magnetic resonance research and electronic paramagnetic resonance research.
- •Research of forces of a superficial tension Properties of liquids
- •Superficial tension.
- •Physical properties of biological membranes.
- •Molecular mechanisms of electrochemical potentials of membranes and distribution of a nervous impulse along excitability fibre.
- •Principles of transformation of biological and not electric signals in electric. Designs of sensors and electrodes, their basic characteristics
- •Application of sensors
- •Analog registering devices
- •Rhythm of heart. Parameters of cardiac activity.
- •External displays of activity of heart.
- •Activity of heart
- •Electric activity of cells of a myocardium
- •Functions of conductive system of heart
- •The electrocardiogram
- •Change of a rhythm of cardiac activity.
- •The device and main principles of work of electrocardiograph
- •Imposing of electrodes
- •The device, a principle of work of electroencephalograph. Basic rhythms of eeg. Registration of eeg and principles of the analysis
- •Introduction
- •Essence of a method
- •Technique of record of electroencephalogram.
- •Principles of analyses of electroencephalogram
- •Definition of a photoeffect
- •Types of photoeffects.
- •Einstein equation
- •Application of a photo effect in medicine.
- •The device, principle of work spectrophotometer. Application spectrophometrical methods of research for definition of concentration of substances in biological liquids
- •Method of the calibre graph.
- •Method of comparison.
- •Polarization of light by biosystems. Polarimeter. Light natural and polarized. Polaroids.
- •Research of microstructures in polarizing light
- •Rotation of a plane of fluctuations of polarized light. Saccharimetry.
- •Design of the device
- •Principle of action
- •Special receptions of microscopy of biological objects Special methods of light microscopy (illumination and supervision). Method of a dark field.
- •Method of ultramicroscopy
- •Method of a light field and its version
- •Method of phase contrast
- •Polarizing microscopy
- •The method interference contrast
- •Method of research in a view of a luminescence
- •Device of a microscope. Measurement of value of microscoping object.
- •Model of sliding strings. Biomechanics of a muscle. The equation of Hill. Modeling of muscular reduction Biophysics of muscular reduction
- •Structure of a cross-section-striped muscle. Model of sliding strands.
- •Biomechanics of a muscle
- •The equation of Hill. Power of single reduction.
- •Modelling of muscular reduction
- •Electromechanical interface in muscles
- •Devices for measurement of function of external breath. The device and a principle of work. Registration and the analysis of the given functional researches
- •Change of saturation by gases of an organism at differences of external pressure.
- •Biomechanics of external breath
- •Devices for measurement of function of external breath
- •Viscosity of a liquid
- •Current of a liquid on a horizontal pipe. Puazal’s law.
- •Current of a liquid on a pipe of variable section and branched out
- •Definition of speed of blood-groove
- •Full resistance (impedance) of tissues of the organism Physical bases of rheography
- •Measurement of factor of viscosity.
- •Physical bases of hemodynamic. Laws of movement of blood in an arterial and venous channel
- •Measurement of pressure of blood
- •Systolic and minute volume of a blood-groove
- •Change of minute volume of a blood-groove at work.
- •Biophysical features of an aorta.
- •Biophysical features of arterioles of the big circle of blood circulation.
- •Tomographic methods
- •Classification of kinds of a tomography.
- •Interposition of a source of probing radiation, bject and the detector.
- •Echosounding
- •Computer tomograph.
- •Magnetic-resonant tomography.
- •Three-dimensional ultrasonic
- •ElastoScan-flexography
- •Modes of an acoustic transparency of three-dimensional ultrasonic.
- •Methods of physiotherapeutic influence
- •The therapeutic techniques based on application of a direct current. Electrophoresis and galvanizing in a medical practice. Mobility of ions.
- •Medicinal electrophoresis.
- •Galvanizing.
- •Electrosecurity
- •The basic requirements to an electrosecurity of electromedical devices and devices.
- •Modelling of biophysical processes.
- •The basic stages of modelling
- •The basic stages of modelling
- •4. Creation of model, its research.
- •Classification of models:
- •Mathematical models of growth of number of a population.
- •Pharmacokinetic model.
- •Sources of X-ray radiation.
- •Artificial sources of radiation.
- •Interaction of X-ray radiation with substance.
- •1. Coherent dispersion;
- •2. Photoeffect
- •3. Kompton - effect
- •Radioactivity radiation Radioactive disintegration Structure of a nucleus
- •A radioactive disintegration. Kinds of radioactive radiation
- •It is thrown out and a nucleus has more stability complex of nucleons. Positron β-disintegration is described by the equation:
- •The basic law of radioactive disintegration in the differential form. A constant of disintegration.
- •The basic law of radioactive disintegration in the differential form.
- •A half-life period.
- •Activity. A unit of measure.
- •Radioactive elements in the nature.
- •Ionizing radioactive radiation and its biological action.
- •The absorbed and exposition doze. Power of a doze. Units of measure.
- •Relative biological efficiency.
- •Mehanisms of action of laser radiation on biological tissues.
- •Mechanisms of action of laser radiation on biological tissues
- •Список использованной литературы :
- •Educational handout on medical biophysics kovaleva larissa valentinovna
Galvanizing.
T
he
medical method, at which action on an organism of a direct current of
insignificant force is used, is
called galvanizing.
It is connected with the old name of a direct current - a galvanic
current. Primary action of a current on tissues of an organism is
connected with movement in tissues of ions of electrolytes and other
charged particles. Division of ions and accordingly, change of
concentration of ions in various elements of tissue structures occurs
owing to different mobility of ions, and as delays and their
accumulation at semipermeable membranes, in tissue elements, outside
and inside of cells. It causes change of a functional condition of a
cell and other physiological processes in fabrics. Therapeutic action
of a direct current depends on this phenomenon. Thus, change of
concentration of ions in fabric formations is a basis of primary
action of a direct current on an organism of the person.
Owing
to different mobility and as presence on semipermeable membranes of
environments, division of ions occurs and accordingly, changes of
concentration in various elements of fabric structures. According to
the ionic theory of irritation of P.P.Lazarev, destruction of the
certain parity of concentration of the ions which are being on both
party of an environment causes a condition of excitation in a cell.
It is reaction to action of an electric current. The attitude of
concentration of monovalent ions Na and K to concentration of
bivalent ions Ca and Mg has major importance (fig. 4)
I
ncrease
of this parity causes reaction of excitation, and downturn - reaction
of braking. In particular action of a current in the field of the
cathode is connected with increase of concentration of more mobile
monovalent ions, mainly K and Na. Increase of excitability in the
field of the anode is connected with concentration of less mobile
bivalent ions Са,
Mg, which remain much near to the anode.
At galvanizing the direct current a pressure 60-80 V, force of a current from 5 up to 15 mА, at the density of a current which are not exceeding 0,1mА/cm2, is brought to tissues by means of wires and soft plastic electrodes. Lead with tin is applied as a material to electrodes. On the one hand it possesses softness, with another - it forms the most inactive ion. Therefore ions of lead do not participate in formation of a current.
I
mposing
of metal electrodes directly on a skin is inadmissible.
Because,
formed on a surface of electrodes products of electrolysis a solution
of the chloride sodium containing in fabrics, the solution of cookery
salt which is being in structure of sweat, possess cauterizing
property and cause burns of a leather.
Thick enough lining (1) (look fig. 5) from a hygroscopic material (from flannels), is moistened with water or a weak salt solution. It absorbs in herself products of secondary reactions on an electrode. This lining keeps within on a surface of a skin under an electrode (2) (fig. 5,6). A lining with an electrode become stronger and densely nestle on a body in the necessary place by means of plaits or elastic bandage (3). Linings are sterilized with boiling and used repeatedly.
Electrodes of corresponding forms and the sizes are used for leading a direct current to a site of a body of the patient. The electrode consists of a metal plate or from a material which well spends an electric current and linings from hydrophilic fabrics thickness not less than 1 сm, which sizes on 1,5 - 2 cm exceed the sizes of a metal plate on all perimeter. The lining placed between a metal plate and a body of the patient, protects a skin from burns and irritations sour and alkaline products of electrolysis. These products are formed on a metal part of electrodes at passage of a current. The multicore isolated wires are applied to connection of electrodes with plugs of the device.
Linings plunge into hot water by preparation for carrying out of medical procedure of galvanizing. Then they are moderately wrung out and imposed on sites of a body a subject influence together with current-carrying plates. Then they are fixed by means of elastic bandage. It is necessary to watch, that linings densely and exactly adjoined to a body. Electrodes should not concern directly bodies. If on a body there are wounds, grazes, they should be covered by a slice of cotton wool or gauze with vaseline.
Electroconductivity of separate sites of the organism which is being between electrodes depends on resistance of skin and hypodermics. The current extends basically on blood and lymphatic vessels, muscles, environments of nervous trunks inside of an organism. Resistance of skin is defined by its condition: thickness, age, humidity. The arrangement of electrodes on a body of the patient can be cross-section, longitudinal or slanting. The distance between the edges of both electrodes turned to each other should be not less width of one of electrodes. Usually electrodes are applied identical on the size. If necessary to strengthen action of a current on a site of a body, the electrode of the smaller area in comparison with the second settles down on it. Flat trays are used for influence on area of fine joints of fingers of brushes and stop. The metal electrode falls to a tray as it is possible further from a site of a body, for exception of casual contact of a body with a metal part of an electrode. The second electrode is located on a hand or a leg of the patient, in cervicobrachial or lumbar area of a spinal.
D
evices
of type «Поток
-1» are applied to galvanizing
The device has the case from the shock-resistant polystyrene, consisting of own case and a demountable bottom. Milliamperemeter (1) (look fig. 3), it is located at the left on the top wall of the case serving by the control panel. The handle of a regulator of a current «3», - to the right of the ammeter - the switch of ranges of a current and limits of measurement milliamperemeter «5 mA – 50 mA» «4», a control bulb «2», network switch " Вкл-Выкл" (5) (it means switch on - switch off), target jacks 6 ("+" - a red key, «-» - a black key). The aperture is in day of the case for access to a safety lock.
Before carrying out of procedures it is necessary to check up correctness of installation of the switch of a pressure of a network. To translate the switch of a network in position «Выкл», the switch of ranges - in position «5 mA», and the handle of adjustment of a current - in zero position. To include a plug of a network cord in the socket of a power line. To connect connecting wires to target clips and to strengthen in their clips the chosen electrodes. To impose on a body of the patient electrodes with the linings moistened by water or a medicinal solution (at carrying out of procedures medicinal electroforesis). To include mains voltage (thus the lamp on the control panel will light up) and, smoothly turning the handle of a regulator, it is necessary to establish value of a current. It is necessary to mean, that within the first minutes after the beginning of procedure resistance of a body decreases a little that leads to increase in a current. For this reason in the beginning of procedure it is necessary to watch size of a current and if necessary to adjust it. For reduction of a range of a current preliminary to deduce in initial position the handle of adjustment of a current and to remove electrodes from the patient. At a break in work to switch off a network feed, having translated the handle of the network switch in position «выкл».
Procedure of the general galvanizing is spent with use of the baths filled by water, into which extremity of the patient plunge.
The current brought to the patient is dosed out on density - to the attitude of force of a current to the area of an electrode. The admissible density of a current at local galvanizing should not exceed 0.1 mA/cm2. At the general influences admissible density of a current on the order below - 0.01 mA/cm2- 0.05 mA/cm2.
Subjective sensations of the patient are considered too. The patient should feel a lung pricking (tingle) under electrodes during procedure. Occurrence of feeling of burning serves as a signal to decrease in density of a spent current.
Inclusion of a current should begin with zero value; accrue very gradually and smoothly, without jerks and pushes. Resistance of a skin at its heating by damp linings decreases also a current gradually increases. Deenergizing of a current should be is spent very smoothly up to zero. Upon termination of procedure the device should be switched off and wires are disconnected from it. Duration of procedures, frequency of carrying out and their general number on course of treatment depends on character, a stage and a phase of disease, the general condition of the patient and its specific features.
I
f
it is necessary to raise concentration of those or other ions in all
organism with this purpose the four-chamber bath is applied.
Galvanizing is combined with a high-frequency magnetotherapy (inductothermy), and acupuncture.
The current brought to the patient is dosed out on density - to the attitude of force of a current to the area of an electrode. The admissible density of a current at local galvanizing should not exceed 0.1 mA / sec2. Admissible density of a current on the order below - 0.01 mA / sec2 - 0.05 mA / sec2 at the general and segmentary influences
Subjective sensations of the patient are considered at batching time of influence of an electric current and its sizes. The patient should feel a pricking (tingle) under electrodes during procedure. Occurrence of feeling of burning serves as a signal to decrease in density of a spent current.
Duration of galvanizing does not exceed 20 - 30 minutes 10 - 15 procedures are appointed usually to course of treatment. If necessary the repeated rate of galvanizing is spent in one month.
To advantages of a method of medicinal electrophoresis is carried: 1. Creation of skin depot in which medicinal substances are found out from 1 till 3 days, 2. Influence is direct on the pathological center, 3. Significant decrease of frequency of physiological reactions, 4. Painless introduction of medicinal substances. Galvanizing is used at treatment - traumas and diseases of peripheral nervous system (radiculitises, neuropathy, neuralgias, etc.), - traumas and diseases of CNS (traumas of spinal, frustration of brain and spinal blood circulation), - a vegetative dystonia, neurasthenias and other neurotic conditions, - diseases of bodies of the digestion, proceeding with infringement motor and secretory functions (chronic gastritises, colitis, cholecystitises, a dyskinesia of bile-excreting ways, a stomach ulcer of a stomach and a duodenal gut), - hypertonic and hypotonic illness, a stenocardia, an atherosclerosis in initial stages, - chronic inflammatory processes in various bodies and fabrics, - chronic arthritises and periarthritis a traumatic, rheumatic and exchange origin. Contra-indications for galvanizing: New growths, sharp inflammatory and purulent processes, system diseases of the blood, sharply expressed atherosclerosis, hypertonic illness of III stage, a feverish condition, eczema, dermatitis, extensive infringements o f integrity of an integument and frustration of skin sensitivity places of imposing of electrodes, propensity to bleedings, pregnancy, individual intolerance of a galvanic current.
Indications to medicinal electrophoresis Are rather wide - they are defined by therapeutic properties of entered preparation with the obligatory account of indications to use of a direct current. It is possible to count on the general action of medicinal substance mainly at functional vegetovascular frustration and conditions at which there is enough microdoze of medicinal substances. Contra-indications to medicinal electrophoresis are the same, as to galvanizing, and also individual intolerance of medicinal substances.
